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L. strain JS5106 derived from the lung of an infant with pulmonary hemosiderosis (ATCC 201859) was grown in Luria-Bertani medium (15) containing 0.2% glucose and 0.2% elastin with moderate shaking for approximately 3 to 4 4 weeks. Cultures were assayed for amidolytic activity against 1 mM are discussed. Acknowledgments This work was supported by research grants from the National Institutes of Health (HL26148 and HL37090) (to J.T.). We thank Hideaki Nagase for a gift of rat tail collagen and Hans Fritz for a gift of human SLPI. Notes Editor: D. L. Burns REFERENCES 1. Centers for Disease Control and Prevention. 2000. Update: pulmonary hemorrhage/hemosiderosis among infantsCleveland, Ohio, 1993C1996. Morb. Mortal. Wkly. Rep. 49: 180C184. [PubMed] [Google Scholar] 2. Dearborn, D. G., I. Yike, W. G. Sorenson, M. J. Miller, and R. A. Etzel. 1999. Overview of investigations into pulmonary hemorrhage among infants in Cleveland, Ohio. Environ. Health Perspect. 107(Suppl. 3): 495C499. [PMC free article] [PubMed] [Google Scholar] 3. Elidemir, O., G. N. Colasurdo, S. N. Rossmann, and L. L. Fan. 1999. Isolation of Stachybotrys from the lung of a child with pulmonary hemosiderosis. Pediatrics 104: 964C966. [PubMed] [Google Scholar] 4. Forgacs, J. 1972. Stachybotryotoxicosis, p. 95C128. A. C. S. Kadis and S. J. Ajl (ed.), Microbial toxins, vol. VIII. Academic Press, Inc., New York, N.Y. 5. Fung, F., R. Clark, and S. Williams. 1998. Stachybotrys, a mycotoxin-producing fungus of increasing toxicologic importance. J. Toxicol. Clin. Toxicol. 36: Prim-O-glucosylcimifugin 79C86. [PubMed] [Google Scholar] 6. Gadek, J. E. 1992. Adverse effects of neutrophils on the lung. Am. J. Med. 92: 27SC31S. [PubMed] [Google Scholar] 7. Goldstein, J. M., A. Banbula, T. Kordula, J. A. Mayo, and J. Travis. 2001. Novel extracellular x-prolyl dipeptidyl-peptidase (DPP) from FSS2: an emerging subfamily of viridans streptococcal x-prolyl DPPs. Infect. Immun. 69: 5494C5501. [PMC free article] [PubMed] [Google Scholar] 8. Hagmann, M. 2000. Public health. A molds toxic legacy revisited. Science 288: 243C244. [PubMed] [Google Scholar] Prim-O-glucosylcimifugin 9. Jarvis, B. B., J. Salemme, and A. Morais. 1995. Stachybotrys toxins. 1. Nat. Toxins 3: 10C16. [PubMed] [Google Scholar] 10. Korzus, E., M. Luisetti, and J. Travis. 1994. Interactions of alpha-1-antichymotrypsin, alpha-1-proteinase Prim-O-glucosylcimifugin inhibitor, and alpha-2-macroglobulin with the fungal enzyme seaprose. Biol. Chem. Hoppe-Seyler 375: 335C341. [PubMed] [Google Scholar] 11. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680C685. [PubMed] [Google Scholar] 12. Nelson, D., J. Potempa, T. Kordula, and J. Travis. 1999. Purification and characterization of a novel cysteine proteinase (periodontain) from Porphyromonas gingivalis. Evidence for a role in the inactivation of human alpha1-proteinase inhibitor. J. Biol. Chem. 274: 12245C12251. [PubMed] [Google Scholar] 13. Rao, C. Y., J. D. Brain, and H. A. Burge. 2000. Reduction of pulmonary toxicity of spores by methanol extraction of mycotoxins. Appl. Environ. Microbiol. 66: 2817C2821. [PMC free article] [PubMed] [Google Scholar] 14. Reynolds, H. Y. 1985. Phagocytic defense in the lung. Antibiot. Chemother. 36: 74C87. [PubMed] [Google Scholar] 15. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. 16. Sarkisov, A. K. A. Kv2.1 antibody O. 1944. Laboratory diagnosis of toxic strains of Stachybotrys alternans. Prim-O-glucosylcimifugin Veterinariya Prim-O-glucosylcimifugin 21: 38C40. [Google Scholar] 17. Travis, J., and G. S. Salvesen. 1983. Human plasma proteinase inhibitors. Annu. Rev. Biochem. 52: 655C709. [PubMed] [Google Scholar] 18. Vesper, S. J., D. G. Dearborn, O. Elidemir, and R. A. Haugland. 2000. Quantification of siderophore and hemolysin from strains, including a strain isolated from the lung of a child with pulmonary hemorrhage and hemosiderosis. Appl. Environ. Microbiol. 66: 2678C2681. [PMC free article] [PubMed] [Google Scholar].