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Pursuing three 5-min washes with PBS, cells had been incubated with secondary antibodies for 20?min in room temp

Pursuing three 5-min washes with PBS, cells had been incubated with secondary antibodies for 20?min in room temp. and HR restoration. Accordingly, lack of AUNIP or ablation of its capability to bind SCH 442416 to DNA leads to cell hypersensitivity toward a number of DSB-inducing agents, the ones that induce replication-associated DSBs particularly. Our findings […]