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On the other hand, overexpression of ClC5 significantly improved the conversion of LC3B-I to LC3B-II and beclin-1 expression induced by BZ (Fig

On the other hand, overexpression of ClC5 significantly improved the conversion of LC3B-I to LC3B-II and beclin-1 expression induced by BZ (Fig. BZ elevated ClC5 proteins appearance in ARH77, U266, and SKO-007 cells. Knockdown of ClC5 with little interfering RNA sensitized cells to BZ treatment, and upregulation of ClC5 induced chemoresistance to BZ. Furthermore, Olmutinib […]

Lentivirus was produced by co-transfecting the packaging plasmids (PSPAX2 and PMD2

Lentivirus was produced by co-transfecting the packaging plasmids (PSPAX2 and PMD2.G) with lentivirus vectors into 293T cells, using the Attractene transfection reagent (Qiagen, Valencia, CA, USA). differential morphological changes could not be induced by ATRA in NB4-R1 cells infected BMS-582949 with P30 expressing or control vector. Thus, we inferred that ATRA sensitivity of NB4-R1 cells […]