After 7 days of incubation, cells were harvested, stained with appropriately labeled mAbs against CD4 (clone RM4-5), CD8 (clone 53-6
After 7 days of incubation, cells were harvested, stained with appropriately labeled mAbs against CD4 (clone RM4-5), CD8 (clone 53-6.7), CD3 (clone 145-2C11), B220 (clone RA3-6B2), Gr1 (clone RB6-8C5), CD11c (clone HL3) all from BD Biosciences, NK1.1 (clone PK136, Sony) and CD11b (clone M1-70), ckit (CD117, clone 2B-8), Sca1(anti-Ly6A/E, clone D7) and PDCA-1 (clone eBio927) […]