One more needle was inserted into each vial in order to avoid creating negative pressure inside the vial. The analysis was performed on a portable electronic nose 3 (PEN 3) magic size e-nose from Airsense Analytics GmbH (Schwerin, Germany). electronic Dexamethasone acetate nose (e-nose) seems to be a encouraging rapid/screening method to detect contamination by aflatoxin, fumonisin, or both in maize kernel stocks. L.) is one of the most important food and feed commodities among cereal plants. However, its diffusion, recognition, and, most of all, safety for usage are threatened by mycotoxin contamination . Maize is definitely often infected by mycotoxigenic fungi, most notably spp. . Since it is definitely impossible to fully eliminate the presence of these undesirable substances and the risks linked to them, maximum suitable concentrations should be arranged at stringent but reasonable-to-achieve levels. Mycotoxin regulations have been founded in more than 100 countries around the world [3,4], and the maximum suitable Dexamethasone acetate limits vary greatly from country to country. The European Union (EU) offers harmonized regulations among its member claims for maximum and/or recommended levels of mycotoxins in both food and feed [5,6]. The purpose is definitely to keep contaminated grain out of the feed and food chains, and to reduce the potential risk associated with mycotoxins. However, more than 300 compounds have been recognized as mycotoxins, of which around 30 are considered a danger to human being or animal health. Furthermore, global studies indicate that more than 70% of the samples of feed and raw feed materials are positive for at least one mycotoxin, emphasizing co-contamination like a big issue for both food safety and general public health [7,8,9]. The co-occurrence of mycotoxins in food and feed is definitely explained by three reasons: (i) Most fungi are able to simultaneously produce several mycotoxins; (ii) commodities can be contaminated by several fungi simultaneously or in quick succession; and (iii) the complete diet comprises different commodities [8,10,11]. Among different mycotoxins, aflatoxin (AF) and fumonisin (FM) contamination are quite common in different world areas . AFs are most often recognized in Southern Europe, Africa, Dexamethasone acetate South Asia and Southeast Asia (average ideals of positive samples higher than 30%). The highest incidence of fumonisin contamination (more than 50% of positive samples) was found in South America, Southern OCLN Europe, Africa, North, and South and Southeast Asia. Although these mycotoxins are quite ubiquitously present in feed materials, the levels of recognized mycotoxins are generally low. Multi-mycotoxin contamination, however, is definitely a topic of great concern, as co-contaminated samples might still exert adverse effects on animals due to additive/synergistic relationships of the mycotoxins. The difficulty of mycotoxin relationships varies according to the animal species, the level and type of mycotoxin contamination and the space of exposure . This scenario underlines the importance of multi-mycotoxin analysis methods that can also become quick and user-friendly. Rapid methods for the dedication of mycotoxins in cereals are hence highly needed in order to prevent the access of mycotoxins into food and feed chains. In this respect, the contribution of commercial enzyme-linked immunosorbent assays (ELISA) and lateral circulation immunoassays (LFIAs) for mycotoxin dedication is definitely important and has been quite well defined . Implementing these assays and combining them with additional methods will also be essential and necessary. Electronic noses (e-nose) may represent a encouraging analytical tool for industry, also in combination with immunoassays. An e-nose consists of an array of nonspecific chemical detectors that detect different volatile organic compounds (VOCs) and consequently provides a transmission that can.