The exsA exsD(exsAD) twice mutant was similarly constructed, except how the downstream flanking series (1,700 bp) was generated using chromosomal DNA from a PA103 exsDmutant (21) as template inside a PCR, and a primer incorporating a BclI site of BamHI was used because the exsDallele provides the second option instead
The exsA exsD(exsAD) twice mutant was similarly constructed, except how the downstream flanking series (1,700 bp) was generated using chromosomal DNA from a PA103 exsDmutant (21) as template inside a PCR, and a primer incorporating a BclI site of BamHI was used because the exsDallele provides the second option instead. sponsor cells, both which result […]